pstat3 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc pstat3

EZH2 methylates and activates STAT3 in podocytes. ( A - B ) Western blotting and quantitative analysis of <t>pSTAT3/STAT3</t> in each group. P values vs. db/m group ( n = 6). ( C - D ) Western blotting and quantitative analysis of pSTAT3/STAT3 in MPCs. ns: P > 0.05 vs. NG group. P values vs. NG group ( n = 3). ( E - F ) Western blotting and quantifications of pSTAT3/STAT3 in different groups. ns: P > 0.05 vs. db/db group. P values vs. db/db group ( n = 6). ( G - H ) Western blotting and quantitative analysis of pSTAT3/STAT3 and H3K27me3 in MPCs. ns: P > 0.05 vs. HG group. P values vs. HG group ( n = 3). ( I ) Co-immunoprecipitation (Co-IP) revealed a relationship between EZH2 and STAT3, as well as EZH2 and pSTAT3. The Input group served as a positive control, and the IgG group served as a negative control. ( J ) Illustrative confocal microscopic images of EZH2 (red) and pSTAT3 (green) in MPCs counterstained with DAPI (blue). Scale bar = 20 μm. ( K ) Image of molecular docking between EZH2 (pink) and STAT3 (green). ( L ) Co-IP showed the interaction between EZH2 and STAT3 after EZH2 inhibition by GSK126. ( M ) The methylation status of lysine residues of STAT3 was evaluated by Co-IP in MPCs treated with NG or HG. ( N ) The methylation status of lysine residues of STAT3 after EZH2 knockdown (KD) by adenovirus

Pstat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat3/Phospho-Stat3+(Tyr705)+Mouse+mAb/pmc13013736-92-14-18
Average 96 stars, based on 337 article reviews

pstat3 - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "Histone methyltransferase EZH2 drives podocyte injury and senescence in diabetic nephropathy through STAT3 activation"

Article Title: Histone methyltransferase EZH2 drives podocyte injury and senescence in diabetic nephropathy through STAT3 activation

Journal: Cellular and Molecular Life Sciences: CMLS

doi: 10.1007/s00018-026-06136-x

EZH2 methylates and activates STAT3 in podocytes. ( A - B ) Western blotting and quantitative analysis of pSTAT3/STAT3 in each group. P values vs. db/m group ( n = 6). ( C - D ) Western blotting and quantitative analysis of pSTAT3/STAT3 in MPCs. ns: P > 0.05 vs. NG group. P values vs. NG group ( n = 3). ( E - F ) Western blotting and quantifications of pSTAT3/STAT3 in different groups. ns: P > 0.05 vs. db/db group. P values vs. db/db group ( n = 6). ( G - H ) Western blotting and quantitative analysis of pSTAT3/STAT3 and H3K27me3 in MPCs. ns: P > 0.05 vs. HG group. P values vs. HG group ( n = 3). ( I ) Co-immunoprecipitation (Co-IP) revealed a relationship between EZH2 and STAT3, as well as EZH2 and pSTAT3. The Input group served as a positive control, and the IgG group served as a negative control. ( J ) Illustrative confocal microscopic images of EZH2 (red) and pSTAT3 (green) in MPCs counterstained with DAPI (blue). Scale bar = 20 μm. ( K ) Image of molecular docking between EZH2 (pink) and STAT3 (green). ( L ) Co-IP showed the interaction between EZH2 and STAT3 after EZH2 inhibition by GSK126. ( M ) The methylation status of lysine residues of STAT3 was evaluated by Co-IP in MPCs treated with NG or HG. ( N ) The methylation status of lysine residues of STAT3 after EZH2 knockdown (KD) by adenovirus

Figure Legend Snippet: EZH2 methylates and activates STAT3 in podocytes. ( A - B ) Western blotting and quantitative analysis of pSTAT3/STAT3 in each group. P values vs. db/m group ( n = 6). ( C - D ) Western blotting and quantitative analysis of pSTAT3/STAT3 in MPCs. ns: P > 0.05 vs. NG group. P values vs. NG group ( n = 3). ( E - F ) Western blotting and quantifications of pSTAT3/STAT3 in different groups. ns: P > 0.05 vs. db/db group. P values vs. db/db group ( n = 6). ( G - H ) Western blotting and quantitative analysis of pSTAT3/STAT3 and H3K27me3 in MPCs. ns: P > 0.05 vs. HG group. P values vs. HG group ( n = 3). ( I ) Co-immunoprecipitation (Co-IP) revealed a relationship between EZH2 and STAT3, as well as EZH2 and pSTAT3. The Input group served as a positive control, and the IgG group served as a negative control. ( J ) Illustrative confocal microscopic images of EZH2 (red) and pSTAT3 (green) in MPCs counterstained with DAPI (blue). Scale bar = 20 μm. ( K ) Image of molecular docking between EZH2 (pink) and STAT3 (green). ( L ) Co-IP showed the interaction between EZH2 and STAT3 after EZH2 inhibition by GSK126. ( M ) The methylation status of lysine residues of STAT3 was evaluated by Co-IP in MPCs treated with NG or HG. ( N ) The methylation status of lysine residues of STAT3 after EZH2 knockdown (KD) by adenovirus

Techniques Used: Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Positive Control, Negative Control, Inhibition, Methylation, Knockdown

Acrylamide Gel Assay:

Article Title: Dietary oleuropein and its acyl derivative ameliorate inflammatory response in peritoneal macrophages from pristane-induced SLE mice via canonical and noncanonical NLRP3 inflammasomes pathway.
Article Snippet: Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content.. The journal’s standard Terms & Conditions and the Ethical guidelines still apply.. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.

Polyacrylamide Gel Electrophoresis:

Membrane:

Incubation:

Double Staining:

Article Title: A novel dietary multifunctional ingredient reduces body weight and improves leptin sensitivity in cafeteria diet-fed rats
Article Snippet: .. Double staining was performed by simultaneous incubation with the following primary antibodies overnight at 4 °C: mouse anti-pSTAT3 (1:100, Cell Signalling Technology Europe, B. V, Leiden, Netherlands) and guinea pig anti-ACTH (1:500, National Hormone & Peptide Program, Los Angeles, CA, USA). .. The sections were then washed and incubated at room temperature for 1 h with goat antimouse Alexa Fluor 488 and goat anti-guinea pig Alexa Fluor 594 (1:200, Thermo Fisher Scientific Inc., Bilbao, Spain).

Staining:

Article Title: The Stat3 inhibitor F0648-0027 is a potential therapeutic against rheumatoid arthritis
Article Snippet: Page 7/21 NIH3T3 cells were xed in 4% paraformaldehyde (Wako Pure Chemical Industries, Osaka, Japan) and then incubated with PBS buffer containing 0.5%Triton. .. Cells were blocked using 5%BSA for 20 minutes at room temperature and then stained with rabbit anti-Stat3 (1:100 dilution; #12640, Cell signaling Technology, Danvers, MA, USA) or mouse anti-pSTAT3 (1:100 dilution; #4113, Cell signaling Technology, Danvers, MA, USA), followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:100 dilution; Invitrogen, Carlsbad, CA, USA) or Alexa Fluor 488 goat anti-mouse IgG (1:100 dilution; Invitrogen, Carlsbad, CA, USA), respectively. .. After staining in secondary antibodies for 1h at room temperature, DAPI (1:1000; Wako Pure Chemicals Industries, Osaka, Japan) was used as a nuclear stain.

Article Title: The Stat3 inhibitor F0648-0027 is a potential therapeutic against rheumatoid arthritis.
Article Snippet: NIH3T3 cells were fixed in 4% paraformaldehyde (Wako Pure Chemical Industries, Osaka, Japan) and then incubated with PBS buffer containing 0.5%Triton. .. Cells were blocked using 5%BSA for 20 min at room temperature and then stained with rabbit antiStat3 (1:100 dilution; #12640, Cell signaling Technology, Danvers, MA, USA) or mouse anti-pSTAT3 (1:100 dilution; #4113, Cell signaling Technology, Danvers, MA, USA), followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:100 dilution; Invitrogen, Carlsbad, CA, USA) or Alexa Fluor 488 goat anti-mouse IgG (1:100 dilution; Invitrogen, Carlsbad, CA, USA), respectively. .. After staining in secondary antibodies for 1h at room temperature, DAPI (1:1000; Wako Pure Chemicals Industries, Osaka, Japan) was used as a nuclear stain.

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